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Title: Development and validation of uv-spectrophotometer analytical method of Eflornithine hydrochloride
Authors: Grewal I K
Singh S
Arora S
Sharma N
Keywords: Accuracy
Eflornithine hydrochloride
Limit of detection
Limit of quantification
Issue Date: 2020
Publisher: Plant Archives
Abstract: The objective of present analysis is development and validation of UV-spectrophotometer analytical technique for quantitative analysis of Eflornithine hydrochloride (EFH). The standard curve was plotted for EFH concentration range of 0.5-4.5 ?g/ml. The analytical method was validated for linearity, accuracy, robustness and intra-day as well as inter-day precision. Limit of detection (LOD) and limit of quantification (LOQ) was determined for sensitivity determination. Regression equation obtained from calibration curve was y = 0.2221 � -0.016. Developed analytical method for EFH was found linear in concentration range of 0.5-4.5 ?g/ml with high correlation coefficient of 0.9992. Mean percentage recovery was in range of 98-102% which validated the accuracy of the technique. Method exhibited robustness, intra-day and inter-day precision as demonstrated by relative standard deviation <2%. LOD and LOQ of EFH were found 0.01716 and 0.052 ?g/ml, respectively. It was concluded that developed UV spectrophotometer method was accurate, precise, linear, robust and sensitive; therefore, it was concluded that developed UV-spectrophotometer methodology using dansyl chloride as derivatizing agent could be employed for regular assessment and quantifiable evaluation of the EFH. � 2020 Plant Archives. All rights reserved.
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