Evaluation of oxidative stress and its modulation by L-Arginine and L-Ascorbic acid in repetitive restraint stress model in Wistar rats

Abstract

Methods In the present animal study restrain stress for 1 h daily for 21 days was used to induce oxidative stress in Wistar rats. Antioxidant effect of L-Arginine and L-Ascorbic acid was evaluated using malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) levels and total antioxidant capacity levels. Result The results of our study demonstrated that repetitive restraint stress significantly enhance MDA levels and suppress SOD, GSH, catalase levels as well as total antioxidant capacity compared to vehicle group. L-Arginine (500 and 1000 mg/kg, I.P.) and L-Ascorbic acid (100 and 200 mg/kg, I.P) reversed changes of serum MDA, GSH, SOD, catalase levels and total antioxidant capacity level. Conclusion Results from our study demonstrated reversal of oxidative stress markers by L-Arginine and L- Ascorbic acid. They may be used therapeutically in disease associated with high oxidative stress.