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Title: Estimation of Delafloxacin Using Derivative Spectrophotometry and Area Under Curve in Bulk Material and in Laboratory Mixture
Authors: Dhangar, Kiran R.
Shirkhedkar, Atul A.
Keywords: Delafloxacin
Zero order Derivative
First order Derivative
Area under curve
Second Order Derivative
Issue Date: 7-May-2016
Publisher: Chitkara University Publications
Abstract: Simple, specific, rapid and accurate UV-spectrophotometric methods have been developed using a solvent acetonitrile (50 %) to determine delafloxacin in bulk material and in laboratory mixture. “Method A” is zero order derivative UV- spectrophotometry using absorbance, “Method B” is zero order derivative UV-spectrophotometry using Area Under Curve (AUC) technique, “Method C” is first order derivative UV-spectrophotometry using amplitude, “Method D” is First Order Derivative UV-spectrophotometry- AUC,“Method E” is Second Order Derivative UV-spectrophotometry using amplitude and “Method F” is second order derivative UV- spectrophotometry using (AUC) technique. The developed methods have shown excellent results in terms of linearity and range, accuracy, precision and Limit of Detection (LOD) and Limit of Quantification (LOQ). In all Methods, delafloxacin obeyed linearity in the concentration range of 2 - 12 μg/mL with (r2 > 0.999). All these methods were applied for estimation of delafloxacin in laboratory mixture. All the above mentioned methods were validated considering linearity and range, accuracy, precision, ruggedness and sensitivity.
ISSN: Print 2321-2217
Online 2321-2225
Appears in Collections:JPTRM Volume 4 Number 1 (May - 2016)

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